Peptide Reconstitution

What you’ll need:

Peptides
Bacteriostatic Water
Syringes
Alcohol Swabs

The Process of Reconstitution

When ready to reconstitute remove your vial from the fridge and pop the top cap; this will reveal a rubber stopper. You may need to do the same for your bacteriostatic water.

You will want to adequately clean the rubber stopper with an alcohol swab; moving back and forth is best.

Once both rubber stoppers have been sanitized proceed to remove both caps on your syringe. This will reveal a plunger handle and needle point.

You are ready to begin. Always be sure to perform said actions in a clean environment, using a fresh needle and having washed your hands.

Before inserting the needle, into the bacteriostatic water, draw up a volume of air by pulling the plunger back. This volume should be equivalent to your desired volume of water.

Puncture the vial of bacteriostatic water, aiming for the center of the rubber stopper. Flip the vial and needle upside down so the needle point is aiming to the sky and the vial top pointing towards the ground; this will ensure you draw water and not air. Push the syringe plunger forward allowing the full volume of air to enter the vial. After the syringe has been emptied of all air… draw the required volume of water in units by pulling the plunger back.

Then removing the syringe from the bacteriostatic water immediately puncture the vial of peptide; again aiming for the center of the rubber stopper.

Press the plunger down, forcing water into the vial. Once contact is made the peptide should dissolve.

After all the water has entered the vial remove the syringe.

With the syringe out of the vial, pull the plunger all the way out; creating a chimney like structure. Re-puncture the vial once more with the plunger removed from the syringe. This will equalize any pressure imbalances. After a second or two remove the syringe and recap the needle end. Dispose of the needle in a safe manner.

Check the vial of peptide to ensure all the powder has dissolved. Rotate or turn the vial to “collect” any amount that has yet to dissolve.

Post reconstitution you will want to keep your vials in a cool, dark environment; refrigeration at 4 degrees C is ideal. This reduces chance of degradation and improves shelf life. Kept within a fridge most will find 4 to 6 weeks without significant degradation. 2 If a fridge is not available you may be able to without significant degradation. 3.

CALCULATIONS

A quick calculation tip. 1 mcg/kg is considered ones situation dose; in reference the GHRP and GHRH usage. 5 Most doses are referred to using this scale (i.e. half saturation, quarter saturation, full saturation). For a 220 lb man 100 mcg of GHRP or GHRH is considered saturation. You will want to reconstitute in a manner that is productive to both your saturation and syringe capability (sometimes syringe units are difficult to asses).

When performing calculations keep in mind that the amount of peptide within your vial is a constant, so long as you do not remove anything from your peptide vial, AND that using less water for reconstitution leads to longer shelf life. 4

Quick Conversions
1 MG = 1,000 MCG
1 ML= 100 Units

To achieve a desired amount per unit use the following egreention:

Amount of total peptide in mcg/ Amount Desired per Unit in mcg= amount of water needed in units

For example.. if you want 50mcg per unit and you have a 5mg vial:

5 mg= 5000 mcg

5,000mcg/ 50mcg per unit= 100 units of water

So you would need to reconstitute using 100 units of water and you would get 50 mcg of peptide per each unit of water. With this you would need 2 units to get 100mcg of peptide… and so fourth.

If you want 20mcg per unit and you have a 5mg vial:

5 mg= 5000 mcg

5,000mcg/ 20mcg per unit= 250 units of water

So you would need to reconstitute using 250 units of water and you would get 20 mcg of peptide per each unit of water. With this you would need 5 units to get 100mcg of peptide… and so fourth.

DRAWING UP PEPTIDE FOR USE

Reconstituted peptide is ready to use. Following your calculations you should know exactly how much peptide is present per unit. Your goal is to inject under the skin, SubQ, and into the fat layer present on the belly face. Avoid areas around the belly button and be sure to inject into an area with some amount of fat present.

Before starting any injection process be sure to have properly assessed your environment and personal cleanliness:  washing your hands, avoiding injections in places such as the restroom and having stored your peptide vial in a sanitary location.

Start by using an alcohol swab to clean the rubber stopper of your peptide vial.

Using a fresh syringe remove both protective ends; revealing both the plunger and the needle.

Insert the needle, as best you can, into the center of the rubber stopper.

Flip the vial and needle upside down so the needle point is aiming to the sky and the vial top pointing towards the ground; this will ensure you draw peptide and not air.

Draw your exact amount, paying attention to your unit increment (does your syringe use 5 increments for 10 units? or 10 increments for 10 units? This is important to address).

After drawing up the exact amount remove your syringe from the peptide vial. With the syringe in hand use your other hand to lift your shirt, revealing your belly, and clean the injection area with an alcohol swab.

Pinch underneath the area you just cleaned, creating a slight bulge, and quickly push the needle into the location.

Once in the proper location you may proceed to push the plunger down, allowing the peptide to enter the injection point.

After a few seconds, allowing the syringe to empty, remove the syringe. Recap and properly dispose.

If using two peptides it is okay to draw up both into the same syringe and perform one injection. When mixed in 1 syringe be sure to use the peptide within a reasonable timeframe; 12 hours is a good number for those whom need to be exact. Keeping peptides in an unmixed state will prevent degradation over the long term, so it’s ideal to only mix when ready to inject.

FOR THOSE NEW TO INJECTION TECHNIQUE

https://www.youtube.com/watch?v=wXjQHAxopzk

 

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